Q.1 Flavr Savr is a popular example of transgenic crop_________.
Potato Tomato Brinjal
Q.2 Pre-zygotic barrier of distant hybridization is overcome by_________.
Anther culture Ovule culture In vitro pollination
Q.3 Resultant plant with a foreign gene is________.
Transgenic plant Transformed plant LMO
Q.4 Most succcessful and widely used fusogen is____________.
NaNO3 Ca+2 PEG
Q.5 DNA directly injected into the plant protoplasts or cells using fine tipped glass needles is_______.
Electroporation Gene gun Microinjection
Q.6 Which method is used for the production of haploids?
Protoplast culture Anther culture Embryo culture
Q.7 Cell without cell wall is known as_________.
Tonoplast Protoplast Chloroplast
Q.8 The father of plant tissue culture is__________.
Kanta Skoog G. Haberlandt
Q.9 Who discovered PCR?
Smith and Natham Maxam and Gilbert Karry Mullis
Q.10 Which dye is used as tracking dye during electrophoresis?
Ethidium bromide Fulgen stain Bromophenol blue
Q.11 The most common type of restriction enzyme used during gene cloning is_______.
Type-I Type-II Type-III
Q.12 Agarose is isolated from_______.
Bacteria Fungus Algae
Q.13 Coding sequence of gene is_________.
Intron Exon Muton
Q.14 Bt stands for_______.
Bacillus thuringiensis Bacillus thermis Bacillus tinctora
Q.15 First generation of transgenic plant is denoted by__________.
T0 T1 R0
Q.16 Metal particle widely used in gene gun method is_________.
Gold Silver Iron
Q.17 The number of plasmid present per bacterial cell is_______.
Complementary number Code number Copy number
Q.18 Western blotting is used for________.
Protein Lipids RNA
Q.19 In vitro amplification of DNA is done by__________.
LCR PCR HPLC
Q.20 The unique pattern of DNA fragments identified by Southern hybridization or PCR is_________.
DNA fingerprinting Protein fingerprinting Radio-labelling
Q.21 Application of an electric current to a plant cell for transforming DNA is________.
Electroporation Transformation Ligation
Q.22 In vitro vegetative propagation is known as______.
Somaclonal variation Micropropagation Embryo rescue
Q.23 The DNA visualized after electrophoresis under U.V radiation is due to________.
Ethidium bromide TBE Chloremphenicol
Q.24 The largest size of DNA fragment can be accommodated in_______.
Phagemid Cosmid YAC
Q.25 The denaturation step in PCR is carried out at________.
94 ºC 37 ºC 105 ºC
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