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Plant Breeding & Genetics-7

Q.1
Flavr Savr is a popular example of transgenic crop_________.

Potato
Tomato
Brinjal


Q.2
Pre-zygotic barrier of distant hybridization is overcome by_________.
Anther culture
Ovule culture
In vitro pollination


Q.3
Resultant plant with a foreign gene is________.
Transgenic plant
Transformed plant
LMO


Q.4
Most succcessful and widely used fusogen is____________.
NaNO3
Ca+2
PEG


Q.5
DNA directly injected into the plant protoplasts or cells using fine tipped glass needles is_______.
Electroporation
Gene gun
Microinjection


Q.6
Which method is used for the production of haploids?
Protoplast culture
Anther culture
Embryo culture

Q.7
Cell without cell wall is known as_________.

Tonoplast
Protoplast
Chloroplast


Q.8
The father of plant tissue culture is__________.
Kanta
Skoog
G. Haberlandt


Q.9
Who discovered PCR?
Smith and Natham
Maxam and Gilbert
Karry Mullis


Q.10
Which dye is used as tracking dye during electrophoresis?
Ethidium bromide
Fulgen stain
Bromophenol blue


Q.11
The most common type of restriction enzyme used during gene cloning is_______.
Type-I
Type-II
Type-III


Q.12
Agarose is isolated from_______.
Bacteria
Fungus
Algae


Q.13
Coding sequence of gene is_________.
Intron
Exon
Muton


Q.14
Bt stands for_______.
Bacillus thuringiensis
Bacillus thermis
Bacillus tinctora


Q.15
First generation of transgenic plant is denoted by__________.
T0
T1
R0


Q.16
Metal particle widely used in gene gun method is_________.
Gold
Silver
Iron


Q.17
The number of plasmid present per bacterial cell is_______.
Complementary number
Code number
Copy number


Q.18
Western blotting is used for________.
Protein
Lipids
RNA


Q.19
In vitro amplification of DNA is done by__________.
LCR
PCR
HPLC


Q.20
The unique pattern of DNA fragments identified by Southern hybridization or PCR is_________.
DNA fingerprinting
Protein fingerprinting
Radio-labelling


Q.21
Application of an electric current to a plant cell for transforming DNA is________.
Electroporation
Transformation
Ligation


Q.22
In vitro vegetative propagation is known as______.
Somaclonal variation
Micropropagation
Embryo rescue


Q.23
The DNA visualized after electrophoresis under U.V radiation is due to________.
Ethidium bromide
TBE
Chloremphenicol


Q.24
The largest size of DNA fragment can be accommodated in_______.
Phagemid
Cosmid
YAC


Q.25
The denaturation step in PCR is carried out at________.
94 ºC
37 ºC
105 ºC

Number of score out of 25 = Score in percentage =


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